Category: 673-22-3

《Visualization of endogenous β-galactosidase activity in living cells and zebrafish with a turn-on near-infrared fluorescent probe》 was published in Talanta in 2020. These research results belong to Pang, Xiao; Li, Yaqian; Zhou, Zile; Lu, Qiujun; Xie, Ruihua; Wu, Cuiyan; Zhang, Youyu; Li, Haitao. Synthetic Route of C8H8O3 The article mentions the following:

β-Galactosidase (β-gal) is an important biomarker for primary ovarian cancers. Developing noninvasive bioimaging probes for studying the activity of β-gal is highly desirable for cancer diagnosis. Herein, a turn-on near-IR (NIR) fluorescent probe, 2-((6-(((2S, 3R, 4S, 5R, 6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran -2-yl)oxy)-2,3-dihydro-1H-xanthen-4-yl)methylene)malononitrile named DXM-βgal, was rationally designed based on enzymic reaction for the detection of β-gal activity both in vitro and in vivo. Upon incubating with β-gal, DXM-βgal displayed a significant fluorescence enhancement at 640 nm, accompanying by a color change of solution color from red to purple. DXM-βgal exhibited high selectivity and sensitively to β-gal with low limit of detection (2.92 x 10-4 U mL-1). Besides, based on its advantages of long-wavelength emission and excellent biocompatibility, DXM-βgal was successfully applied to imaging β-gal in living cells and zebrafish. Given these prominent properties, we believe that DXM-βgal will be a potential tool for investigating β-gal activity in biomedical research. The results came from multiple reactions, including the reaction of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Synthetic Route of C8H8O3)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Synthetic Route of C8H8O3

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

《An APN-activated NIR photosensitizer for cancer photodynamic therapy and fluorescence imaging》 was published in Biomaterials in 2020. These research results belong to Zhou, Xiao; Li, Haidong; Shi, Chao; Xu, Feng; Zhang, Zhen; Yao, Qichao; Ma, He; Sun, Wen; Shao, Kun; Du, Jianjun; Long, Saran; Fan, Jiangli; Wang, Jingyun; Peng, Xiaojun. Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde The article mentions the following:

Photodynamic therapy has been developed as a prospective cancer treatment in recent years. Nevertheless, conventional photosensitizers suffer from lacking recognition and specificity to tumors, which causing severe side effects to normal tissues, while the enzyme-activated photosensitizers are capable of solving these conundrums due to high selectivity towards tumors. APN (Aminopeptidase N, APN/CD13), a tumor marker, has become a crucial targeting substance owing to its highly expressed on the cell membrane surface in various tumors, which has become a key point in the research of anti-tumor drug and fluorescence probe. Based on it, herein an APN-activated near-IR (NIR) photosensitizer (APN-CyI) for tumor imaging and photodynamic therapy has been firstly developed and successfully applied in vitro and in vivo. Studies showed that APN-CyI could be activated by APN in tumor cells, hydrolyzed to fluorescent CyI-OH, which specifically located in mitochondria in cancer cells and exhibited a high singlet oxygen yield under NIR irradiation, and efficiently induced cancer cell apoptosis. Dramatically, the in vivo assays on Balb/c mice showed that APN-CyI could achieve NIR fluorescence imaging (λem = 717 nm) for endogenous APN in tumors and possessed an efficient tumor suppression effect under NIR irradiation The experimental process involved the reaction of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

Synthetic Route of C8H8O3In 2021 ,《A ratiometric fluorescent probe for the rapid and specific detection of HSO3- in water samples》 appeared in Luminescence. The author of the article were Mu, Xinyue; Zhu, Jinbiao; Yan, Liqiang; Tang, Ningli. The article conveys some information:

Hydrosulphite (HSO3-), as a common and important chem. reagent, is widely used in everyday life, however excessive use and abuse of HSO3- can cause potential harmful effects on the environment and in biol. health. In this paper, we describe the design and preparation of a colorimetric and ratiometric fluorescence probe for the visual detection of HSO3- (excitation wavelengths were, resp., 336 nm and 520 nm). This method showed some advantages including simple preparation, high selectivity, fast response, and significant color and fluorescence ratio (F450/F594) changes in the presence of HSO3-. In addition, this probe was used successfully for the detection of HSO3- in real water samples and showed a good recovery rate range. In the experimental materials used by the author, we found 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Synthetic Route of C8H8O3)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Synthetic Route of C8H8O3

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

In 2019,RSC Advances included an article by Sakai, Rika; Iguchi, Hiroki; Maruyama, Tatsuo. Formula: C8H8O3. The article was titled 《Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound》. The information in the text is summarized as follows:

Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer. In the experimental materials used by the author, we found 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Formula: C8H8O3)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Formula: C8H8O3

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

The author of 《Visible-Light-Induced Regioselective Alkylation of Coumarins via Decarboxylative Coupling with N-Hydroxyphthalimide Esters》 were Jin, Can; Yan, Zhiyang; Sun, Bin; Yang, Jin. And the article was published in Organic Letters in 2019. Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde The author mentioned the following in the article:

An efficient photocatalytic decarboxylative 3-position alkylation of coumarins by using alkyl N-hydroxyphthalimide esters as alkylation reagents has been developed. A variety of NHP esters derived from aliphatic carboxylic acids (primary, secondary, and tertiary) has been proved to be tolerated for this decarboxylation process, affording a broad scope of 3-alkylated coumarin derivatives in moderate to excellent yields. This protocol was highlighted by its mild conditions, readily available starting materials, operational simplicity, and wide functional group tolerance. The experimental part of the paper was very detailed, including the reaction process of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Application In Synthesis of 2-Hydroxy-4-methoxybenzaldehyde

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

In 2019,Tetrahedron included an article by Yahaya, Issah; Seferoglu, Nurgul; Seferoglu, Zeynel. Reference of 2-Hydroxy-4-methoxybenzaldehyde. The article was titled 《Improved one-pot synthetic conditions for synthesis of functionalized fluorescent coumarin-thiophene hybrids: Syntheses, DFT studies, photophysical and thermal properties》. The information in the text is summarized as follows:

We have synthesized coumarin-thiophene hybrids using stepwise and one-pot methods under MWI and CM conditions, and then report the findings and conclusions: one-pot is better than stepwise in terms of high yields and fastness of getting products, and MWI (microwave irradiation) is better than CM (conventional method) in terms of purity of compounds, better yields, and reaction period. Even though both protocols were found to be efficient, the microwave-assisted irradiation reactions were identified to be better, environmentally friendly, cost-effective, mild, more efficient, less time consuming but with high purity. The two protocols were employed for the synthesis of the target coumarin-thiophene hybrids via the Gewald reaction in stepwise and in one-pot three-component. The dyes were prepared in good to excellent yields. Although, we recorded better yields via the stepwise protocol (80-96%), for the synthesis of the target mols., as compared to the one-pot (82-90%), the stepwise condition is fraught with many changes such as long reaction conditions, extra use of solvents for purification of intermediates, and long reaction durations. Photophys. activities of all the target derivatives were investigated using a combination of UV-vis and fluorescence spectroscopies in different solvents. The umbelliferon-like derivative 3g (I) was further investigated for possible use as a pH sensor via deprotonation (using TBAOH) and reverse protonation (using TFA), and was found to be applicable as pH sensor both in organic medium (DMSO) and partial aqueous binary (DMSO/H2O, volume/volume, 9:1) medium. Again, the ground and the excited state properties of the umbelliferon-like derivative 3g and its deprotonated form were also studied using the quantum chem. calculations Finally, the thermal properties of all the target compounds were investigated using TGA in order to test their potency and applicability as optical dyes. All the dyes 3a-j have high thermal stability therefore they can be applicable as optical dyes. The experimental part of the paper was very detailed, including the reaction process of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Reference of 2-Hydroxy-4-methoxybenzaldehyde)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Reference of 2-Hydroxy-4-methoxybenzaldehyde

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

In 2022,Li, Bai; Wang, Minghui; Gu, Xin; Chen, Jiajia; Yang, Xindi; Liu, Xuejin; Xu, Kuoxi published an article in Microchimica Acta. The title of the article was 《A hemicyanidin-based NIR fluorescent probe for detection of H2S and imaging study in cells and mice》.Recommanded Product: 2-Hydroxy-4-methoxybenzaldehyde The author mentioned the following in the article:

The selective detection of hydrogen sulfide in physiol. and pathol. processes has gained substantial attention in recent years. However, the real-time detection of hydrogen sulfide remains an elusive goal. In this work, a new type of hemicyanidin-based fluorescent “”turn-on”” probe NTR-HS (Ex = 680 nm, Em = 760 nm) was developed to detected H2S in a very short time (3 min). The fluorescence quantum yield is 0.15 and accompanied with a noticeable color change from violet to blue that can be used to detect H2S in the range 1.04 x 10-7-4 x 10-5 M with a limit of detection of 1.04 x 10-7 M. The NTR-HS probe was also used for imaging of endogenous hydrogen sulfide and mitochondrial localization in HCT116 and HeLa cells. The detection mechanism was studied through fluorescence, UV-Vis, NMR, and mass anal. Notably, the probe was successfully used to imaging H2S in mice and locating hydrogen sulfide in the large intestine of mice. In addition to this study using 2-Hydroxy-4-methoxybenzaldehyde, there are many other studies that have used 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Recommanded Product: 2-Hydroxy-4-methoxybenzaldehyde) was used in this study.

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Recommanded Product: 2-Hydroxy-4-methoxybenzaldehyde

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

In 2022,Li, Xiang; Shen, Yang; Zhang, Guodong; Zheng, Xin; Zhao, Qing; Song, Zihe published an article in Organic Letters. The title of the article was 《Ru(II)-Catalyzed Decarbonylative Alkylation and Annulations of Benzaldehydes with Iodonium Ylides under Chelation Assistance》.Safety of 2-Hydroxy-4-methoxybenzaldehyde The author mentioned the following in the article:

A Ru(II)-catalyzed decarbonylative alkylation and annulation of salicylaldehydes and 2-aminobenzaldehydes with iodonium ylides has been developed for the synthesis of dibenzo[b,d]furans and NH-free carbazolones. The reaction proceeds smoothly under mild conditions with a low catalyst loading and a broad substrate compatibility. Notably, hydroxy and free amino groups were demonstrated to be the effective directing groups, enabling the successful aldehyde C-H bond activation and subsequent decarbonylation and annulation under the inexpensive Ru(II) catalyst. The experimental process involved the reaction of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Safety of 2-Hydroxy-4-methoxybenzaldehyde)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Safety of 2-Hydroxy-4-methoxybenzaldehyde

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

《A hemicyanine-based “”turn-on”” fluorescent probe for the selective detection of Cu2+ ions and imaging in living cells》 was written by Li, Bai; Kou, Jiejian; Mei, Huihui; Gu, Xin; Wang, Minghui; Xie, Xinmei; Xu, Kuoxi. Recommanded Product: 673-22-3 And the article was included in Analytical Methods in 2020. The article conveys some information:

A novel hemicyanine-based fluorescent probe was developed for the fast, selective, and reversible detection of Cu2+ ions with “”turn-on”” fluorescence response in the aqueous solution The sensing mode was demonstrated through fluorescence, UV-vis, HRMS, 1H NMR, and DFT calculations The probe was successfully used for the imaging of Cu2+ ions in living cells. In the experimental materials used by the author, we found 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Recommanded Product: 673-22-3)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is employed in the synthesis of Schiff base ligand. It is applied as a reactant in the synthesis of LPA1R antagonists used in the inhibition of LPA-induced proliferation and contraction of normal human lung fibroblasts. Also used in the synthesis of tyrosine kinase 6 proteinase inhibitors.Recommanded Product: 673-22-3

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem

Younes, Maged; Aquilina, Gabriele; Castle, Laurence; Engel, Karl-Heinz; Fowler, Paul; Frutos Fernandez, Maria Jose; Fuerst, Peter; Guertler, Rainer; Gundert-Remy, Ursula; Husoey, Trine; Manco, Melania; Moldeus, Peter; Passamonti, Sabina; Shah, Romina; Waalkens-Berendsen, Ine; Wolfle, Detlef; Wright, Matthew; Benigni, Romualdo; Chipman, Kevin; Cordelli, Eugenia; Degen, Gisela; Carfi, Maria; Vianello, Giorgia; Mennes, Wim published an article in 2021. The article was titled 《Scientific opinion on flavoring group evaluation 414 (FGE .414): 2-hydroxy-4-methoxybenzaldehyde》, and you may find the article in EFSA Journal.Product Details of 673-22-3 The information in the text is summarized as follows:

The EFSA Panel on Food Additives and Flavorings (FAF) was requested to evaluate the safety of the substance 2-hydroxy-4-methoxybenzaldehyde [FL -no: 05.229] as a new flavoring substance, in accordance with Regulation (EC) No 1331/2008. 2-Hydroxy-4-methoxybenzaldehyde belongs to chem. group 23 (Commission Regulation (EC) No 1565/2000) and is structurally related to the hydroxy- and alkoxy-ring substituted benzyl derivatives evaluated in FGE .52 and in FGE .20Rev4. The Panel considered the structural/metabolic similarity sufficient to evaluate the candidate substance following a group-based approach according to the EFSA Guidance on the data required for the risk assessment of flavorings to be used in or on foods. The information provided on the manufacturing process, the composition and the stability of [FL -no: 05.229] was considered sufficient. From studies carried out with this substance, the Panel concluded that there is no concern with respect to genotoxicity. Based on QSAR evaluation of possible metabolism, and based on information from structurally related substances, various metabolic routes can be anticipated, which only result in the formation of innocuous metabolites. The exposure estimates for [FL -no: 05.229] (24 and 60 μg/person per day for children and adults, resp.) were below the Threshold of Toxicol. Concern (TTC) for its structural class (I). Accordingly, toxicity studies are not required and the Panel concluded at step A3 of the Procedure that 2-hydroxy-4-methoxybenzaldehyde is not of safety concern when used as a flavoring substance at the intended uses and use levels. Cumulative exposure estimates for 2-hydroxy-4-methoxybenzaldehyde and three structurally related substances (2.4 and 6.2 mg/kg body weight (bw) per day for adults and children, resp.) are above the TTC for structural class I, but below the ADI (acceptable daily intake) of 0-10 mg/kg bw per day for vanillin, which is one of these structurally related substances. Therefore, the cumulative exposure to these four substances [FL -no: 05.015, 05.018, 05.229 and 09.749] also does not raise a safety concern. After reading the article, we found that the author used 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Product Details of 673-22-3)

2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Product Details of 673-22-3

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem