In 2018,Yao, Hong; Wei, Guoxiang; Liu, Yanpeng; Yao, Hequan; Zhu, Zheying; Ye, Wencai; Wu, Xiaoming; Xu, Jinyi; Xu, Shengtao published 《Synthesis, Biological Evaluation of Fluorescent 23-Hydroxybetulinic Acid Probes, and Their Cellular Localization Studies》.ACS Medicinal Chemistry Letters published the findings.COA of Formula: C9H19NO4 The information in the text is summarized as follows:
23-Hydroxybetulinic acid (23-HBA) is a complex lupane triterpenoid, which has attracted increasing attention as an anticancer agent. However, its detailed mechanism of anticancer action remains elusive so far. To reveal its anticancer mode of action, a series of fluorescent 23-HBA derivatives conjugated with coumarin dyes were designed, synthesized, and evaluated for their antiproliferative activities. Subcellular localization and uptake profile studies of representative fluorescent 23-HBA probe 26c were performed in B16F10 cells, and the results suggested that probe 26c was rapidly taken up into B10F10 cells in a dose-dependent manner and mitochondrion was the main site of its accumulation. Further mode of action studies implied that the mitochondrial pathway was involved in 23-HBA-mediated apoptosis. Together, our results provided new clues for revealing the mol. mechanism of natural product 23-HBA for its further development into an antitumor agent. In the experimental materials used by the author, we found tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6COA of Formula: C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Although ethers resist hydrolysis, they are cleaved by hydrobromic acid and hydroiodic acid. Hydrogen chloride cleaves ethers only slowly. COA of Formula: C9H19NO4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem