In 2018,Agrawalla, Bikram Keshari; Wang, Tao; Riegger, Andreas; Domogalla, Matthias P.; Steinbrink, Kerstin; Doerfler, Thilo; Chen, Xi; Boldt, Felix; Lamla, Markus; Michaelis, Jens; Kuan, Seah Ling; Weil, Tanja published 《Chemoselective Dual Labeling of Native and Recombinant Proteins》.Bioconjugate Chemistry published the findings.Name: tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate The information in the text is summarized as follows:
The attachment of two different functionalities in a site-selective fashion represents a great challenge in protein chem. The authors report site specific dual functionalization of peptides and proteins capitalizing on reactivity differences of cysteines in their free (thiol) and protected, oxidized (disulfide) forms. The dual functionalization of interleukin 2 and EYFP proceeded with no loss of bioactivity in a stepwise fashion applying maleimide and disulfide rebridging allyl-sulfone groups. In order to ensure broader applicability of the functionalization strategy, a novel, short peptide sequence that introduces a disulfide bridge was designed and site-selective dual labeling in the presence of biogenic groups was successfully demonstrated. After reading the article, we found that the author used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Name: tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Oxygen is more electronegative than carbon, thus the alpha hydrogens of ethers are more acidic than those of simple hydrocarbons. They are far less acidic than alpha hydrogens of carbonyl groups (such as in ketones or aldehydes), however. Name: tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem