In 2018,Journal of the American Chemical Society included an article by Liu, Yu; Wolstenholme, Charles H.; Carter, Gregory C.; Liu, Hongbin; Hu, Hang; Grainger, Leeann S.; Miao, Kun; Fares, Matthew; Hoelzel, Conner A.; Yennawar, Hemant P.; Ning, Gang; Du, Manyu; Bai, Lu; Li, Xiaosong; Zhang, Xin. Product Details of 139115-91-6. The article was titled 《Modulation of Fluorescent Protein Chromophores To Detect Protein Aggregation with Turn-On Fluorescence》. The information in the text is summarized as follows:
We present a fluorogenic method to visualize misfolding and aggregation of a specific protein-of-interest in live cells using structurally modulated fluorescent protein chromophores. Combining photophys. anal., X-ray crystallog., and theor. calculation, we show that fluorescence is triggered by inhibition of twisted-intramol. charge transfer of these fluorophores in the rigid microenvironment of viscous solvent or protein aggregates. Bioorthogonal conjugation of the fluorophore to Halo-tag fused protein-of-interests allows for fluorogenic detection of both misfolded and aggregated species in live cells. Unlike other methods, our method is capable of detecting previously invisible misfolded soluble proteins. This work provides the first application of fluorescent protein chromophores to detect protein conformational collapse in live cells. In the experiment, the researchers used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Product Details of 139115-91-6)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Oxygen is more electronegative than carbon, thus the alpha hydrogens of ethers are more acidic than those of simple hydrocarbons. They are far less acidic than alpha hydrogens of carbonyl groups (such as in ketones or aldehydes), however. Product Details of 139115-91-6
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem