The author of 《A novel near-infrared fluorescent probe for detecting intracellular alkaline phosphatase and imaging of living cells》 were Xu, Longbin; He, Xu; Huang, Yibing; Ma, Pinyi; Jiang, Yanxiao; Liu, Xin; Tao, Shuo; Sun, Ying; Song, Daqian; Wang, Xinghua. And the article was published in Journal of Materials Chemistry B: Materials for Biology and Medicine in 2019. Electric Literature of C8H8O3 The author mentioned the following in the article:
Development of novel near-IR (NIR) fluorescent probes for the monitoring of active substances in living organism is desirable in biol. studies. Herein, we designed a novel NIR MTR fluorophore which has a longer emission wavelength, greater Stokes shift and higher quantum yield than the classic hemicyanine NIR fluorophore. The synthesized MTR-derived NIR probe (MTR-P, I) is highly selective and sensitive to alk. phosphatase (ALP) activity. In the presence of ALP, MTR-P exhibited increased fluorescence signal by up to 56 fold at 723 nm, and it was determined to be 0.042 U L-1. In addition, the mechanism of the MTR-P probe was further examined by HPLC, mass spectrometry and DFT/TDDFT calculations The NIR probe MTR-P was successfully applied to measure the levels of alk. phosphatase in different types of cells by fluorescence imaging. The experimental process involved the reaction of 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Electric Literature of C8H8O3)
2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Electric Literature of C8H8O3
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