Ratiometric quantitation of thiol metabolites using non-isotopic mass tags was written by Zhao, Xiaofeng;Hui, Dawn S.;Lee, Richard;Edwards, James L.. And the article was included in Analytica Chimica Acta in 2018.Formula: C9H10O4 This article mentions the following:
Ratiometric quantitation was used in mass spectrometry to account for variations in ionization efficiencies due to heterogenous sample matrixes. Isotopes are most commonly used to achieve ratiometric quantitation because of their ability to coelute chromatog. with each other and to have similar ionization efficiencies. In the work presented here, a new non-isotopic quant. tagging approach is presented which allows chromatog. coelution and similar ionization efficiencies. Using two variations of maleimide tags, t-Bu and cyclohexyl maleimide, thiols are quantified with a high degree of linearity up to five-fold concentration differences. Because these two tags have similar hydrophobcities, they elute simultaneously which allows them to be used for ratiometric quantitation. Beyond the five-fold linear range, signal compression is observed This technique was able to quantify thiol changes in both in vitro pharmacol. treatments as well as in vivo diabetic tissue. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Formula: C9H10O4).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Ethers are good solvents partly because they are not very reactive. Most ethers can be cleaved, however, by hydrobromic acid (HBr) to give alkyl bromides or by hydroiodic acid (HI) to give alkyl iodides. Ethers can form hydrogen bonds with other molecules (alcohols, amines, etc.) that have O―H or N―H bonds. The ability to form hydrogen bonds with other compounds makes ethers particularly good solvents for a wide variety of organic compounds and a surprisingly large number of inorganic compounds.Formula: C9H10O4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem