Xu, Hanqing published the artcileConstructing an MCF-7 breast cancer cell-based transient transfection assay for screening RARα (Ant)agonistic activities of emerging phenolic compounds, Application of 4-Hydroxy-3-tert-butylanisole, the main research area is phenolic compound RARalpha antagonist transfection assay breast carcinoma cell; (Ant)agonistic activity; Emerging chemicals of concern; Endocrine disrupting effects; Retinoic acid receptor α (RARα); Transient transfection.
The screening of compounds with endocrine disrupting effects has been attracting increasing attention due to the continuous release of emerging chems. into the environment. Testing the (ant)agonistic activities of these chems. on the retinoic acid receptor α (RARα), a vital nuclear receptor, is necessary to explain their perturbation in the endocrine system in vivo. In the present study, MCF-7 breast carcinoma cells were transiently transfected with a RARα expression vector (pEF1α-RARα-RFP) and a reporter vector containing a retinoic acid reaction element (pRARE-TA-Luc). Under optimized conditions, the performance of the newly constructed system was evaluated for its feasibility in screening the (ant)agonistic effects of emerging phenolic compounds on RARα. The results showed that this transient transfection cell model responded well to stimulation with (ant)agonists of RARα, and the EC50 and IC50 values were 0.87 nM and 2.67μM for AM580 and Ro41-5253, resp. Its application in testing several emerging phenolic compounds revealed that triclosan (TCS) and tetrabromobisphenol A (TBBPA) exerted notable RARα antagonistic activities. This newly developed bioassay based on MCF-7 is promising in identifying the agonistic or antagonistic activities of xenobiotics on RARα and has good potential for studying RARα signaling-involved toxicol. effects of emerging chems. of concern.
Journal of Hazardous Materials published new progress about Crystal structure. 121-00-6 belongs to class ethers-buliding-blocks, name is 4-Hydroxy-3-tert-butylanisole, and the molecular formula is C11H16O2, Application of 4-Hydroxy-3-tert-butylanisole.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem