Transcriptome analysis reveals the molecular mechanism of Yiqi Rougan decoction in reducing CCl4-induced liver fbrosis in rats was written by Xiong, Yu;Hu, Jinyuan;Xuan, Chen;Tian, Jiayu;Tan, Kaiyue;Chen, Zhiwei;Luo, Yan;Du, Xuqin;Cheng, Junxiong;Zhang, Lanyue;Cao, Wenfu. And the article was included in Chinese Medicine (London, United Kingdom) in 2021.Recommanded Product: Bisdemethoxycurcumin The following contents are mentioned in the article:
Liver fibrosis develops from various chronic liver diseases, and there is currently a lack of specific treatment strategies. Yiqi Rougan decoction (YQRG) is a traditional Chinese medicine that has shown durative effects in the treatment of liver fibrosis; however, the mechanism associated with YQRG-related improvements in liver fibrosis remains to be exptl. determined This study evaluated the therapeutic effect of YQRG on carbon tetrachloride (CCl4)-induced liver fibrosis in rats and its mol. mechanism. We used low-, medium-, and high-dose YQRG to treat CCl4-induced liver fibrosis in rats, followed by assessment of liver injury and fibrosis according to liver appearance, body weight, liver mass index, histopathol. examination, and serum testing. Addnl., we performed transcriptome anal. using RNA-sequencing (RNA-seq) technol., including cluster, Gene Ontol. (GO), and pathway analyses, to identify differentially expressed genes (DEGs), and protein and gene expression were detected by immunofluorescence (IFC), western blot and real-time quant. PCR. The results showed that YQRG effectively alleviated CCl4-induced liver injury and fibrosis in rats, including observations of improved liver function, decreased activity of hepatic stellate cells (HSCs), and decreased extracellular matrix (ECM) deposition. Moreover, we identified downregulated and upregulated DEGs in the model group relative to the control and YQRG-treated groups, with GO anal. revealing their enrichment in biol. processes, such as endoplasmic reticulum stress (ERS), apoptosis, and autophagy. Furthermore, pathway anal. showed that YQRG treatment downregulated the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/Akt (PI3K/AKT) signalling pathways and upregulated other signalling pathways, including those related to peroxisome proliferator-activated receptors(PPAR) and AMP-activated protein kinase(AMPK), with these findings subsequently verified exptl. These findings showed that YQRG improved CCl4-induced liver fibrosis through multiple mechanisms and pathways, offering critical insight into the YQRG-related therapeutic mechanism and promoting further research into its potential application. This study involved multiple reactions and reactants, such as Bisdemethoxycurcumin (cas: 33171-05-0Recommanded Product: Bisdemethoxycurcumin).
Bisdemethoxycurcumin (cas: 33171-05-0) belongs to ethers. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Recommanded Product: Bisdemethoxycurcumin
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