Synthetic Route of C9H19NO4In 2017 ,《Assaying RNA Localization in Situ with Spatially Restricted Nucleobase Oxidation》 was published in ACS Chemical Biology. The article was written by Li, Ying; Aggarwal, Mahima B.; Nguyen, Kim; Ke, Ke; Spitale, Robert C.. The article contains the following contents:
The authors report herein a novel chem.-genetic method for assaying RNA localization within living cells. RNA localization is critical for normal physiol. as well as the onset of cancer and neurodegenerative disorders. Despite its importance, there is a real lack of chem. methods to directly assay RNA localization with high resolution in living cells. The authors’ novel approach relies on in situ nucleobase oxidation by singlet oxygen generated from spatially confined fluorophores. The authors’ novel method can identify RNA mols. localized within specific cellular compartments. The authors anticipate that this platform will provide the community with a much-needed methodol. for tracking RNA localization within living cells and set the stage for systematic large scale anal. of RNA localization in living systems. In the experiment, the researchers used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Synthetic Route of C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Ethers do have nonbonding electron pairs on their oxygen atoms, and they can form hydrogen bonds with other molecules (alcohols, amines, etc.) that have O―H or N―H bonds. The ability to form hydrogen bonds with other compounds makes ethers particularly good solvents for a wide variety of organic compounds and a surprisingly large number of inorganic compounds.Synthetic Route of C9H19NO4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem