Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces was written by Swainsbury, David J. K.;Harniman, Robert L.;Di Bartolo, Natalie D.;Liu, Juntai;Harper, William F. M.;Corrie, Alexander S.;Jones, Michael R.. And the article was included in Biochimica et Biophysica Acta, Bioenergetics in 2016.Synthetic Route of C9H10O4 This article mentions the following:
A challenge associated with the utilization of bioenergetic proteins in new, synthetic energy transducing systems is achieving efficient and predictable self-assembly of individual components, both natural and man-made, into a functioning macromol. system. Despite progress with water-soluble proteins, the challenge of programming self-assembly of integral membrane proteins into non-native macromol. architectures remains largely unexplored. In this work it is shown that the assembly of dimers, trimers or tetramers of the naturally monomeric purple bacterial reaction center can be directed by augmentation with an 浼?helical peptide that self-associates into extra-membrane coiled-coil bundle. Despite this induced oligomerization the assembled reaction centers displayed normal spectroscopic properties, implying preserved structural and functional integrity. Mixing of two reaction centers modified with mutually complementary 浼?helical peptides enabled the assembly of heterodimers in vitro, pointing to a generic strategy for assembling hetero-oligomeric complexes from diverse modified or synthetic components. Addition of two coiled-coil peptides per reaction center monomer was also tolerated despite the challenge presented to the pigment-protein assembly machinery of introducing multiple self-associating sequences. These findings point to a generalized approach where oligomers or longer range assemblies of multiple light harvesting and/or redox proteins can be constructed in a manner that can be genetically-encoded, enabling the construction of new, designed bioenergetic systems in vivo or in vitro. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Synthetic Route of C9H10O4).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Relative to alcohols, ethers are generally less dense, are less soluble in water, and have lower boiling points. They are relatively unreactive. Ethers can form hydrogen bonds with other molecules (alcohols, amines, etc.) that have O閳ユ椊 or N閳ユ椊 bonds. The ability to form hydrogen bonds with other compounds makes ethers particularly good solvents for a wide variety of organic compounds and a surprisingly large number of inorganic compounds.Synthetic Route of C9H10O4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem