Cytotoxic activity of alkaloids extracted from Trigonella foenum graecum (Fenugreek) against breast cancer cell line was written by Al-Sallami, Alaauldeen S. M.;Al-Labban, Zuhair Salih;Ali, Sanaa Mohemmed. And the article was included in Journal of Pharmaceutical Sciences and Research in 2019.Computed Properties of C12H17NO2 This article mentions the following:
The present study was investigated to Screening for cytotoxic activity of total alkaloid extracts of Trigonella foenum graecum (Fenugreek) against breast cancer cell line Ahmed-Mohammed-Nahi,2003 (AMN-3), AMJ-13 and normal cell line Rat Embryo Fibroblast (REF). The Trigonella foenum powders were extracted sep. with 80% Ethanol and total alkaloids separated at pH 2 and 10 with HCl and ammonia. Total alkaloids were detected qual. by Mayer閳ョ灚, Hager閳ョ灚, and Dragendorff閳ョ灚 reagents. The cytotoxic activity was evaluated by crystal violet assay. The results show that The extract of Trigonella foenum graecum had total alkaloid in extract about 1.7% resulting from getting 4.4 g of dry alkaloid extract from 252 g of dry powder of Trigonella foenum – graecum. The alkaloids reduced the cell viability of all cell lines AMN-3,AMJ-13 and REF, and the inhibitory concentration 50% of cells were 1029 娓璯/mL, 1069 娓璯/mL and 1724 娓璯/mL for AMN-3, AMJ13 and REF resp. Trigonella foenum alkaloids showed variable cytotoxic activity against cancer and normal cell lines depending on the alkaloid concentrations and cell line types. In the experiment, the researchers used many compounds, for example, N-(3-Methoxyphenyl)pivalamide (cas: 56619-93-3Computed Properties of C12H17NO2).
N-(3-Methoxyphenyl)pivalamide (cas: 56619-93-3) belongs to ethers. Relative to alcohols, ethers are generally less dense, are less soluble in water, and have lower boiling points. They are relatively unreactive. Ethers are good solvents partly because they are not very reactive. Most ethers can be cleaved, however, by hydrobromic acid (HBr) to give alkyl bromides or by hydroiodic acid (HI) to give alkyl iodides.Computed Properties of C12H17NO2
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem