Li, Qing Xiao et al. published their research in Journal of Agricultural and Food Chemistry in 1991 | CAS: 5367-32-8

3-Methyl-4-nitroanisole (cas: 5367-32-8) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. But ether is more polar than alkenes. Ethers feature bent C–O–C linkages. In dimethyl ether, the bond angle is 111° and C–O distances are 141 pm. The barrier to rotation about the C–O bonds is low. The bonding of oxygen in ethers, alcohols, and water is similar. In the language of valence bond theory, the hybridization at oxygen is sp3.Safety of 3-Methyl-4-nitroanisole

Development of enzyme-linked immunosorbent assays for 4-nitrophenol and substituted 4-nitrophenols was written by Li, Qing Xiao;Zhao, Meng Shu;Gee, Shirley J.;Kurth, Mark J.;Seiber, James N.;Hammock, Bruce D.. And the article was included in Journal of Agricultural and Food Chemistry in 1991.Safety of 3-Methyl-4-nitroanisole This article mentions the following:

An ELISA was developed for the determination of 4-nitrophenol and monosubstituted 4-nitrophenols such as 2-chloro-4-nitrophenol and 3-methyl-4-nitrophenol. An antiserum (Ab1812) was selected among eight antisera against three different corresponding immunogens. 4-(Nitrophenyl)acetyl OVA conjugate (C-OVA) as a coating antigen was selected among eight hapten-protein conjugates. Using an Ab1812 dilution of 1/1000, I50 values for 4-nitrophenol, 3-methyl-4-nitrophenol, 2-chloro-4-nitrophenol, and 2-amino-4-nitrophenol were 59, 64, 31, and 57 nM, resp. There were no cross-reactivities with other substituted phenols/nitrobenzenes and 2- or 3-nitrophenol. The detection limit of this ELISA was 1000 times lower than that of a standard colorimetric method for the anal. of 4-nitrophenol. Under the optimized condition, this procedure can be used to determine the concentration of 4-nitrophenol and(or) monosubstituted 4-nitrophenols at levels as low as 0.2 ppb in different water samples without extraction and cleanup. In the experiment, the researchers used many compounds, for example, 3-Methyl-4-nitroanisole (cas: 5367-32-8Safety of 3-Methyl-4-nitroanisole).

3-Methyl-4-nitroanisole (cas: 5367-32-8) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. But ether is more polar than alkenes. Ethers feature bent C–O–C linkages. In dimethyl ether, the bond angle is 111° and C–O distances are 141 pm. The barrier to rotation about the C–O bonds is low. The bonding of oxygen in ethers, alcohols, and water is similar. In the language of valence bond theory, the hybridization at oxygen is sp3.Safety of 3-Methyl-4-nitroanisole

Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem