Substrate specificity and properties of the aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii was written by Guillen, Francisco;Martinez, Angel T.;Martinez, Maria Jesus. And the article was included in European Journal of Biochemistry in 1992.Related Products of 3929-47-3 This article mentions the following:
The production in a 5-1 fermenter of the extracellular enzymes laccase and aryl-alc. oxidase by the fungus P. eryngii was studied. The enzyme has been purified 50-fold by Sephacryl S-200 and Mono Q chromatog. Purified aryl-alc. oxidase is a unique flavoprotein with 15% carbohydrate content, a mol. mass of 72.6 kDa (SDS-PAGE) and a pI of 3.9. The enzyme presents wide specificity, showing activity on benzyl, cinnamyl, naphthyl, and aliphatic unsaturated alcs. Neither activity nor inhibition of veratryl alc. oxidation was found with saturated alcs., but competitive inhibition was produced by aromatic compounds which were not aryl-alc. oxidase substrates, such as phenol or 3-phenyl-1-propanol. From these results, it was apparent that a double bond conjugated with a primary alc. is necessary for substrate recognition by aryl-alc. oxidase, and that activity is increased by the presence of addnl. conjugated double bonds and electron donor groups. Both affinity and maximal velocity during enzymic oxidation of methoxybenzyl alcs. were affected in a similar way by ring substituents, increasing from benzyl alc. (Km = 0.84 mM, Vmax = 52 U/mg) to 4-methoxybenzyl alc. (Km = 0.04 mM, Vmax = 208 U/mg). Aryl-alc. oxidase presents also a low oxidase activity with aromatic aldehydes, but the highest activity was found in the presence of electron-withdrawing groups. In the experiment, the researchers used many compounds, for example, 3-(3,4-Dimethoxyphenyl)propan-1-ol (cas: 3929-47-3Related Products of 3929-47-3).
3-(3,4-Dimethoxyphenyl)propan-1-ol (cas: 3929-47-3) belongs to ethers. Of all the functional groups, ethers are the least reactive ones. Ether bonds are quite stable towards bases, oxidizing agents and reducing agents. But on the other hand, ethers undergo cleavage by reaction with acids. Electron-deficient reagents are also stabilized by ethers. For example, borane (BH3) is a useful reagent for making alcohols. Pure borane exists as its dimer, diborane (B2H6), a toxic gas that is inconvenient and hazardous to use. Borane forms stable complexes with ethers, however, and it is often supplied and used as its liquid complex with tetrahydrofuran (THF).Related Products of 3929-47-3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem