Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog was written by Kodan, Atsushi;Yamaguchi, Tomohiro;Nakatsu, Toru;Sakiyama, Keita;Hipolito, Christopher J.;Fujioka, Akane;Hirokane, Ryo;Ikeguchi, Keiji;Watanabe, Bunta;Hiratake, Jun;Kimura, Yasuhisa;Suga, Hiroaki;Ueda, Kazumitsu;Kato, Hiroaki. And the article was included in Proceedings of the National Academy of Sciences of the United States of America in 2014.Related Products of 3929-47-3 This article mentions the following:
P-glycoprotein is an ATP-binding cassette multidrug transporter that actively transports chem. diverse substrates across the lipid bilayer. The precise mol. mechanism underlying transport is not fully understood. Here, the authors present crystal structures of a eukaryotic P-glycoprotein homolog, CmABCB1 with ATPase activity from Cyanidioschyzon merolae, in 2 forms: unbound at 2.6-Å resolution and bound to a unique allosteric peptide inhibitor at 2.4-Å resolution The inhibitor clamped the transmembrane helixes from the outside, fixing the CmABCB1 structure in an inward-open conformation similar to the unbound structure, confirming that an outward-opening motion is required for the ATP hydrolysis cycle. These structures, along with site-directed mutagenesis and transporter activity measurements, revealed the detailed architecture of the transporter, including a gate that opens to extracellular side and 2 gates that open to intramembranous region and the cytosolic side. The authors propose that the motion of the nucleotide-binding domain drives those gating apparatuses via 2 short intracellular helixes, IH1 and IH2, and 2 transmembrane helixes, TM2 and TM5. In the experiment, the researchers used many compounds, for example, 3-(3,4-Dimethoxyphenyl)propan-1-ol (cas: 3929-47-3Related Products of 3929-47-3).
3-(3,4-Dimethoxyphenyl)propan-1-ol (cas: 3929-47-3) belongs to ethers. Of all the functional groups, ethers are the least reactive ones. Ether bonds are quite stable towards bases, oxidizing agents and reducing agents. Ethers are good solvents partly because they are not very reactive. Most ethers can be cleaved, however, by hydrobromic acid (HBr) to give alkyl bromides or by hydroiodic acid (HI) to give alkyl iodides.Related Products of 3929-47-3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem