Phytoene desaturase from Oryza sativa: oligomeric assembly, membrane association and preliminary 3D-analysis was written by Gemmecker, Sandra;Schaub, Patrick;Koschmieder, Julian;Brausemann, Anton;Drepper, Friedel;Rodriguez-Franco, Marta;Ghisla, Sandro;Warscheid, Bettina;Einsle, Oliver;Beyer, Peter. And the article was included in PLoS One in 2015.Related Products of 605-94-7 This article mentions the following:
Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown to be enzymically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by mol. oxygen, serve as a final electron acceptor defining PDS as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. The herbicidal PDS-inhibitor norflurazon is capable of arresting the reaction by stabilizing the intermediary FADred, while an excess of the quinone acceptor relieves this blockage, indicating competition. The enzyme requires its homo-oligomeric association for activity. The sum of data collected through gel permeation chromatog., non-denaturing polyacrylamide electrophoresis, chem. crosslinking, mass spectrometry and electronmicroscopy techniques indicate that the high-order oligomers formed in solution are the basis for an active preparation Of these, a tetramer consisting of dimers represents the active unit. This is corroborated by our preliminary x-ray structural anal. that also revealed similarities of the protein fold with the sequence-inhomologous bacterial phytoene desaturase CRTI and other oxidoreductases of the GR2-family of flavoproteins. This points to an evolutionary relatedness of CRTI and PDS yielding different carotene desaturation sequences based on homologous protein folds. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Related Products of 605-94-7).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Of all the functional groups, ethers are the least reactive ones. Ether bonds are quite stable towards bases, oxidizing agents and reducing agents. Ethers feature bent C–O–C linkages. In dimethyl ether, the bond angle is 111° and C–O distances are 141 pm. The barrier to rotation about the C–O bonds is low. The bonding of oxygen in ethers, alcohols, and water is similar. In the language of valence bond theory, the hybridization at oxygen is sp3.Related Products of 605-94-7
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem