Alkyne-Tag Raman Imaging for Visualization of Mobile Small Molecules in Live Cells was written by Yamakoshi, Hiroyuki;Dodo, Kosuke;Palonpon, Almar;Ando, Jun;Fujita, Katsumasa;Kawata, Satoshi;Sodeoka, Mikiko. And the article was included in Journal of the American Chemical Society in 2012.Computed Properties of C9H10O4 This article mentions the following:
Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous mol. in live cells. Here, we show that alkyne-tag Raman imaging (ATRI) is a promising approach for visualizing nonimmobilized small mols. in live cells. An examination of structure-Raman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogs in live cells. Cellular concentrations of diyne-tagged CoQ analogs could be semiquant. estimated Finally, simultaneous imaging of two small mols., 5-ethynyl-2′-deoxyuridine (EdU) and a CoQ analog, with distinct Raman tags was demonstrated. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Computed Properties of C9H10O4).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. But ether is more polar than alkenes. At room temperature, ethers are pleasant-smelling colourless liquids. Relative to alcohols, ethers are generally less dense, are less soluble in water, and have lower boiling points. They are relatively unreactive, and as a result they are useful as solvents for fats, oils, waxes, perfumes, resins, dyes, gums, and hydrocarbons. Vapours of certain ethers are used as insecticides, miticides, and fumigants for soil.Computed Properties of C9H10O4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem