Activity and stability of uricase from Lactobacillus plantarum immobilized on natural zeolite for uric acid biosensor was written by Iswantini, Dyah;Nurhidayat, Novik;Trivadila;Widiyatmoko, Okik. And the article was included in Pakistan Journal of Biological Sciences in 2014.Related Products of 605-94-7 This article mentions the following:
Determination of uric acid concentration in human urine and blood is needed to diagnose several diseases, especially the occurrence of kidney disease in gout patients. Therefore, it is needed to develop a simple and inexpensive method for uric acid detection. The purpose of the research was to observe the use of Indonesian microbe that was immobilized on natural zeolite as a source of uricase for uric acid biosensor. Selection of mediators and determination of optimum condition measurement, the stability and kinetic properties of L. plantarum uricase were performed using carbon paste electrode. Cyclic voltammetry was employed to investigate the catalytic behavior of the biosensor. The result indicated that the best mediator for measurement of L. plantarum uricase activity was Qo (2,3-dimethoxy-5-methyl-1,4 benzoquinone). Optimum conditions for immobilization of L. plantarum uricase on zeolite were obtained at pH 7.6, with temperature of 28°C, using uric acid concentration of 0.015 mM and zeolite mass at 135 mg KM and VMax of L. plantarum uricase obtained from Lineweaver-burk equation for the immobilization uricase on zeolite were 8.6728 × 10-4 mM and 6.3052 mM, resp. KM value of L. plantarum uricase directly immobilized onto the electrode surface was smaller than KM value of L. plantarum uricase immobilized on zeolite. The smaller KM value shows the higher affinity toward the substrate. The electrode when kept at 10°C was stable until 6 days, however the immobilized electrode on zeolite was stable until 18 days. Therefore, Indonesian L. plantarum could be used as a uric acid biosensor. In the experiment, the researchers used many compounds, for example, 2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7Related Products of 605-94-7).
2,3-Dimethoxy-5-methylcyclohexa-2,5-diene-1,4-dione (cas: 605-94-7) belongs to ethers. Ether is less polar than esters, alcohols or amines because of the oxygen atom that is unable to participate in hydrogen bonding due to the presence of bulky alkyl groups on both sides of the oxygen atom. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly autoxidize to form hydroperoxides and dialkyl peroxides. If concentrated or heated, these peroxides may explode. To prevent such explosions, ethers should be obtained in small quantities, kept in tightly sealed containers, and used promptly.Related Products of 605-94-7
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem