Kikuchi, Arizumi published the artcileEvaluation of the efficacy of various reagents in improving microRNA extraction, SDS of cas: 143-24-8, the main research area is efficacy formalin fixation paraffin embedding microRNA extraction; Efficacy; RNA extraction; formalin fixation and paraffin embedding; microRNA; quantitative real-time reverse transcription PCR; stem-loop RT primer.
Background: MicroRNA has received considerable attention in the clin. context, and attempts are being made to use microRNA in clin. diagnosis. However, adequate quantities of microRNA required for anal. are challenging to isolate. We tested the effect of various reagents in improving microRNA extraction and compared their efficacy to that of a com. available extraction kit (HighPure miRNA isolation kit, Roche). Methods: We used the synthetic oligonucleotide miR-21 and formalin-fixed, paraffin-embedded (FFPE) tissue sections from colon cancer samples (n = 10). We tested increasing volumes (100-600μL) of 1,4-dioxane, 2-butanol, 2-propanol, acetonitrile, polyethylene glycol (PEG) 600, PEG 1000, PEG 1540, PEG 2000, tetraethylene glycol di-Me ether (TDE), and THF, instead of the binding enhancer solution provided in the kit. MiR-21 anal. was performed via stem-loop RT-qPCR using Universal ProbeLibrary probe (Roche). Results: The optimum amount of each enhancement solution was 200-500μL. We obtained ΔCp values of optimum addnl. volume for each solution from 1.04 to 2.50 and compared these with those obtained using the com. available kit. PEG 1540 and 2000 produced superior reactivity with minimal addition For FFPE tissue samples, addition of the enhancement solutions PEG 1540 and 2000 resulted in mean crossing point values of 18.15 ± 2.26 and 17.73 ± 3.26, resp. We obtained a crossing point value of 20.56 ± 4.26 (mean ± SD) using the com. available kit. Conclusions: The tested enhancer reagents, which are relatively readily available and easy to use, can improve microRNA extraction efficacy of a com. available kit.
Annals of Clinical Biochemistry published new progress about Colon neoplasm. 143-24-8 belongs to class ethers-buliding-blocks, name is 2,5,8,11,14-Pentaoxapentadecane, and the molecular formula is C10H22O5, SDS of cas: 143-24-8.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem