Asano, Yasuhisa published the artcileMicrobial degradation of nitrile compounds. Part V. Aliphatic nitrile hydratase from Arthrobacter sp. J-1. Purification and characterization, COA of Formula: C3H7NO2, the main research area is aliphatic nitrile hydratase Arthrobacter.
Aliphatic nitrile hydratase (I) was purified ∼290-fold with a yield of 10% from the cell-free extract of acetonitrile-grown Arthrobacter species J-1. Purified I was homogeneous by ultracentrifugation and disc gel electrophoresis. I catalyzed the stoichiometric hydration of acetonitrile to form acetamide. I was inducibly formed and then amidase, which hydrolyzed acetamide, was formed. The mol. weight of I was ∼420,000 by gel filtration. I was composed of 2 kinds of subunits with mol. weights of 24,000 and 27,000. The isoelec. point was 3.6. I was active toward low-mol.-weight of 2-5 C atoms. The Km for acetonitrile was 5.78 mM. I was inactivated by SH-group reagents and competitively inhibited by KCN with a Ki of 1.5 μM.
Agricultural and Biological Chemistry published new progress about Arthrobacter. 16332-06-2 belongs to class ethers-buliding-blocks, name is 2-Methoxyacetamide, and the molecular formula is C3H7NO2, COA of Formula: C3H7NO2.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem