Identification of Bacillus strains producing glycosidases active on rutin and grape glycosidic aroma precursors was written by Haure, Maxime;Chi Nguyen, Thi Kim;Cendres, Aurelie;Perino, Sandrine;Wache, Yves;Licandro, Helene. And the article was included in LWT–Food Science and Technology in 2022.Computed Properties of C9H12O3 The following contents are mentioned in the article:
Most of the glycosidic aroma precursors in grape consist of diglycosides such as rutinoside. Yeast glycosidases have been extensively studied but their activity is limited to monoglucosidic precursors. Yet, there is no study about application of glycosidases active on rutinoside in grape. In this study, we screened Bacillus isolates for their glycosidase activities on rutin and grape aroma precursors. One Bacillus amyloliquefaciens and one Bacillus licheniformis strains produced extracellular glycosidases, active on both rutin and para-nitrophenol-α-L-rhamnoside. The specific rutinosidic activities were 6.2 and 4.1 pkat/mg resp., and supernatants were active on Muscat grape glycosidic aroma precursors. Both strains were able to release monoterpenoids and benzenoids. The most released monoterpenoid in grape pomace was neric acid, with 378μg/kg and the most released benzenoid was 2-phenylethanol, with 189μg/kg for B. licheniformis. These glycosidases are interesting tools to target rutinosidic glycosides in grape and can be applied as a new way for valorising grape pomace. This study involved multiple reactions and reactants, such as 4-Hydroxy-3-methoxyphenethanol (cas: 2380-78-1Computed Properties of C9H12O3).
4-Hydroxy-3-methoxyphenethanol (cas: 2380-78-1) belongs to ethers. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Computed Properties of C9H12O3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem