Synthesis and biological evaluation of celastrol derivatives as potent antitumor agents with STAT3 inhibition was written by Xu, Shaohua;Fan, Ruolan;Wang, Lu;He, Weishen;Ge, Haixia;Chen, Hailan;Xu, Wen;Zhang, Jian;Xu, Wei;Feng, Yaqian;Fan, Zhimin. And the article was included in Journal of Enzyme Inhibition and Medicinal Chemistry in 2022.Formula: C3H9NO The following contents are mentioned in the article:
Using STAT3 inhibitors as a potential strategy in cancer therapy have attracted much attention. Recently, celastrol has been reported that it could directly bind to and suppress the activity of STAT3 in the cardiac dysfunction model. To explore more effective STAT3 inhibiting anti-tumor drug candidates, we synthesized a series of celastrol derivatives and biol. evaluated them with several human cancer cell lines. The western blotting anal. showed that compound3-Hydroxy-9b,13a-dimethyl-2-oxo-24,25,26-trinoroleana-1(10),3,5,7-tetraen-29-oic acid 2-(2-thienyl) ethylamide,the most active derivative, could suppress the STAT3s phosphorylation as well as its downstream genes. SPR anal., mol. docking and dynamics simulations results indicated that the 3-Hydroxy-9b,13a-dimethyl-2-oxo-24,25,26-trinoroleana-1(10),3,5,7-tetraen-29-oic acid 2-(2-thienyl) ethylamide could bind with STAT3 protein more tightly than celastrol. Then we found that the 3-Hydroxy-9b,13a-dimethyl-2-oxo-24,25,26-trinoroleana-1(10),3,5,7-tetraen-29-oic acid 2-(2-thienyl) ethylamide could block cell-cycle and induce apoptosis on HCT-116 cells. Furthermore, the anti-tumor effect of 3-Hydroxy-9b,13a-dimethyl-2-oxo-24,25,26-trinoroleana-1(10),3,5,7-tetraen-29-oic acid 2-(2-thienyl) ethylamide was verified on colorectal cancer organoid. This is the first research that discovered effective STAT3 inhibitors as potent anti-tumor agents from celastrol derivatives This study involved multiple reactions and reactants, such as 2-Methoxyethylamine (cas: 109-85-3Formula: C3H9NO).
2-Methoxyethylamine (cas: 109-85-3) belongs to ethers. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Esterification is the general name for a chemical reaction in which two reactants (typically an alcohol and an acid) form an ester as the reaction product. Esters are common in organic chemistry and biological materials.Formula: C3H9NO
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem