A new synthetic biology approach for the production of curcumin and its glucoside in Atropa belladonna hairy roots was written by Singh, Sailendra;Pandey, Pallavi;Akhtar, Qussen Md.;Negi, Arvind Singh;Banerjee, Suchitra. And the article was included in Journal of Biotechnology in 2021.Application In Synthesis of Bisdemethoxycurcumin The following contents are mentioned in the article:
Curcumin has ignited global interest as an elite drugable mol., owing to its time-honoured pharmacol. activities against diverse human ailments. Limited natural accessibility and poor oral bioavailability caused major hurdles in the curcumin-based drug development process. We report the first successful testimony of curcumin and its glucoside synthesis in Atropa belladonna hairy roots (HR) through metabolic engineering. Re-routing the inherent biosynthetic precursors of the phenylpropanoid pathway of A. belladonna by heterologous expression of key curcumin biosynthetic pathway genes (i.e., Diketide-CoA synthase-DCS and Curcumin synthase-CURS3) and glucosyltransferase gene (CaUGT2) resulted in the production of curcumin and its glucoside in HR clones. Under shake-flask cultivation, the PGD2-HR1clone bearing DCS/ CURS3 genes showed the maximum curcumin yield (180.62 ± 4.7 μg/g DW), while the highest content of curcumin monoglucoside (32.63 ± 2.27 μg/g DW) along with curcumin (67.89 ± 2.56 μg/g DW) were noted in the PGD3-HR3 clone co-expressing DCS/CURS3 and CaUGT2 genes. Bioreactor up-scaling showed yield improvements in the PGD2-HR1 (2.3 fold curcumin) and the PGD3-HR3 clone (0.9 and 1.65 folds of curcumin-monoglucoside and curcumin resp.). These findings proved the advantageous use of HR cultures as the production source for curcumin and its glucoside, which remained unexplored so far. This study involved multiple reactions and reactants, such as Bisdemethoxycurcumin (cas: 33171-05-0Application In Synthesis of Bisdemethoxycurcumin).
Bisdemethoxycurcumin (cas: 33171-05-0) belongs to ethers. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits, including apples, durians, pears, bananas, pineapples, and strawberries. Esters are more polar than ethers but less polar than alcohols. They participate in hydrogen bonds as hydrogen-bond acceptors, but cannot act as hydrogen-bond donors, unlike their parent alcohols. This ability to participate in hydrogen bonding confers some water-solubility.Application In Synthesis of Bisdemethoxycurcumin
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem