HPLC fingerprint and UV-Vis spectroscopy coupled with chemometrics for Curcumae radix species discrimination and three bioactive compounds prediction was written by Wang, Le;Ren, Xueyang;Wang, Yu;Liu, Xiaoyun;Dong, Ying;Ma, Jiamu;Song, Ruolan;Yu, AXiang;Wei, Jing;Fan, Qiqi;Wang, Xiuhuan;She, Gaimei. And the article was included in Microchemical Journal in 2021.Electric Literature of C19H16O4 The following contents are mentioned in the article:
Curcumae Radix with four botanical origins (Wenyujin, Huangyujin, Guiyujin, Lvyujin) has a long medical history in China. The four species have not been differentiated well in clin. application, though there were differences in pharmacol. effects among them. For the aim of fast discrimination and quant. anal. of Yujin, HPLC (high performance liquid chromatog.) fingerprint and UV-Vis (UV-visible) spectroscopy technologies coupled with chemometrics were employed. The HPLC fingerprints of 26 Guiyujin, 11 Huangyujin, 11 Lvyujin, 29 Wenyujin and full 77 samples were sep. established, with good similarities among same species except Wenyujin and poor consistence in full samples. UV-Vis spectra showed obvious three absorption peaks in the spectra of Huangyujin samples and only one in that of the rest samples. LDA (linear discriminant anal.) and Bagged Trees models established based on HPLC fingerprint data attained higher than 90% discrimination accuracy. Based on the spectral data, complete separation for the four species of Yujin was failed to achieve by PCA (principal component anal.), and the KNN (k-nearest neighbors) and SVM (support vector machine) models achieved no<90% discrimination accuracies. Furthermore, the SVMR (support vector machine regression) and Bagged Trees Regression models used to quickly predict the contents of germacrone, curcumenol and curdione performed well, with relatively lower RMSE (root mean square error) and higher R2 (coefficient of determination) values. The present study proposed relatively rapid methods for the determination of bioactive compounds and discrimination of Yujin. This study involved multiple reactions and reactants, such as Bisdemethoxycurcumin (cas: 33171-05-0Electric Literature of C19H16O4).
Bisdemethoxycurcumin (cas: 33171-05-0) belongs to ethers. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Many esters have the potential for conformational isomerism, but they tend to adopt an s-cis (or Z) conformation rather than the s-trans (or E) alternative, due to a combination of hyperconjugation and dipole minimization effects. The preference for the Z conformation is influenced by the nature of the substituents and solvent, if present. Lactones with small rings are restricted to the s-trans (i.e. E) conformation due to their cyclic structure.Electric Literature of C19H16O4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem