《TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis》 was written by Lee, Po-Hsien; Huang, Xi Xiao; Teh, Bin Tean; Ng, Ley-Moy. COA of Formula: C28H28O3This research focused onTSA CRAFT software boltzmann equation; automatic Tm analysis; differential scanning fluorimetry; high-throughput ligand screening; thermal shift assay; thermofluor. The article conveys some information:
Thermal shift assay (TSA) is an increasingly popular technique used for identifying protein stabilizing conditions or interacting ligands in X-ray crystallog. and drug discovery applications. Although the setting up and running of TSA reactions is a relatively simple process, the subsequent anal. of TSA data, especially in high-throughput format, requires substantial amount of effort if conducted manually. We therefore developed the Thermal Shift Assay-Curve Rapid and Automatic Fitting Tool (TSA-CRAFT), a freely available software that enable automatic anal. of TSA data of any throughput. TSA-CRAFT directly reads real-time PCR instrument data files and displays the analyzed results in a web browser. This software features streamlined data processing and Boltzmann equation fitting, which is demonstrated in this study to provide more accurate data anal. than the commonly used first-derivative method. TSA-CRAFT is freely available as a cross-operating system-compatible standalone tool and also as a freely accessible web. In the part of experimental materials, we found many familiar compounds, such as 6-(3-(Adamantan-1-yl)-4-methoxyphenyl)-2-naphthoic acid(cas: 106685-40-9COA of Formula: C28H28O3)
6-(3-(Adamantan-1-yl)-4-methoxyphenyl)-2-naphthoic acid(cas:106685-40-9) is a third-generation synthetic retinoid and a highly lipophilic compound derived from naphthoic acid. It is widely used in the treatment of acne.COA of Formula: C28H28O3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem