In 2017,Zhang, Huaiying; Aonbangkhen, Chanat; Tarasovetc, Ekaterina V.; Ballister, Edward R.; Chenoweth, David M.; Lampson, Michael A. published 《Optogenetic control of kinetochore function》.Nature Chemical Biology published the findings.SDS of cas: 139115-91-6 The information in the text is summarized as follows:
Kinetochores act as hubs for multiple activities during cell division, including microtubule interactions and spindle checkpoint signaling. Each kinetochore can act autonomously, and activities change rapidly as proteins are recruited to, or removed from, kinetochores. Understanding this dynamic system requires tools that can manipulate kinetochores on biol. relevant temporal and spatial scales. Optogenetic approaches have the potential to provide temporal and spatial control with mol. specificity. Here we report new chem. inducers of protein dimerization that allow us to both recruit proteins to and release them from kinetochores using light. We use these dimerizers to manipulate checkpoint signaling and mol. motor activity. Our findings demonstrate specialized properties of the CENP-E (kinesin-7) motor for directional chromosome transport to the spindle equator and for maintenance of metaphase alignment. This work establishes a foundation for optogenetic control of kinetochore function, which is broadly applicable to exptl. probing of other dynamic cellular processes. After reading the article, we found that the author used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6SDS of cas: 139115-91-6)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers. Ethers lack the hydroxyl groups of alcohols. SDS of cas: 139115-91-6 Without the strongly polarized O―H bond, ether molecules cannot engage in hydrogen bonding with each other.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem