In 2016,Yamaura, Kei; Kiyonaka, Shigeki; Numata, Tomohiro; Inoue, Ryuji; Hamachi, Itaru published 《Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry》.Nature Chemical Biology published the findings.Electric Literature of C9H19NO4 The information in the text is summarized as follows:
The fast inhibitory actions of γ-aminobutyric acid (GABA) are mainly mediated by GABAA receptors (GABAARs) in the brain. The existence of multiple ligand-binding sites and a lack of structural information have hampered the efficient screening of drugs capable of acting on GABAARs. The authors have developed semisynthetic fluorescent biosensors for orthosteric and allosteric GABAAR ligands on live cells via coupling of affinity-based chem. labeling reagents to a bimol. fluorescence quenching and recovery system. These biosensors were amenable to the high-throughput screening of a chem. library, leading to the discovery of new small mols. capable of interacting with GABAARs. Electrophysiol. measurements revealed that one hit, 4,4′,4”-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), was a novel neg. allosteric modulator capable of strongly suppressing GABA-induced chloride currents. Thus, these semisynthetic biosensors represent versatile platforms for screening drugs to treat GABAAR-related neurol. disorders, and this strategy can be extended to structurally complicated membrane proteins. After reading the article, we found that the author used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Electric Literature of C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.The C-O bonds that comprise simple ethers are strong. Electric Literature of C9H19NO4 They are unreactive toward all but the strongest bases. Although generally of low chemical reactivity, they are more reactive than alkanes.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem