In 2015,Li, Daliang; Huang, ZhiJiang; Chen, Shiuhwei; Hu, Zeping; Li, Wen-hong published 《GLP-1 Receptor Mediated Targeting of a Fluorescent Zn2+ Sensor to Beta Cell Surface for Imaging Insulin/Zn2+ Release》.Bioconjugate Chemistry published the findings.Synthetic Route of C9H19NO4 The information in the text is summarized as follows:
The pancreatic islet beta cell plays an essential role in maintaining the normal blood glucose level by releasing insulin. Loss of functional beta cell mass leads to diabetes, a disease affecting ∼9% of the population worldwide. There has been great interest and intense effort in developing imaging probes for monitoring islet beta cells, and glucagon-like peptide-1 receptor (GLP-1R) has emerged as a valuable biomarker for targeting beta cells. However, efforts thus far in GLP-1R mediated beta cell labeling and imaging has largely, if not exclusively, focused on developing imaging probes for monitoring beta cell mass, and few studies have investigated imaging beta cell function (insulin release) through GLP-1R. We now report the design and synthesis of a bioconjugate, ZIMIR-Ex4(9-39), that consists of a fluorescent Zn2+ sensor and a truncated exendin 4 peptide for imaging insulin/Zn2+ release in islet beta cells. In vitro, the conjugate bound to Zn2+ with high affinity and displayed a robust fluorescence enhancement upon Zn2+ chelation. When added to beta cells at submicromolar concentration, ZIMIR-Ex4(9-39) rapidly labeled cell surface in minutes to report the dynamics of insulin/Zn2+ release with high spatiotemporal resolution Future explorations of this approach may lead to probes for tracking beta cell function using different imaging modalities. The experimental process involved the reaction of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Synthetic Route of C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Although ethers resist hydrolysis, they are cleaved by hydrobromic acid and hydroiodic acid. Hydrogen chloride cleaves ethers only slowly. Synthetic Route of C9H19NO4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem