COA of Formula: C9H19NO4In 2018 ,《Development of Chemical Proteomics for the Folateome and Analysis of the Kinetoplastid Folateome》 appeared in ACS Infectious Diseases. The author of the article were Webster, Lauren A.; Thomas, Michael; Urbaniak, Michael; Wyllie, Susan; Ong, Han; Tinti, Michele; Fairlamb, Alan H.; Boesche, Markus; Ghidelli-Disse, Sonja; Drewes, Gerard; Gilbert, Ian H.. The article conveys some information:
The folate pathway has been extensively studied in a number of organisms, with its essentiality exploited by a number of drugs. However, there has been little success in developing drugs that target folate metabolism in the kinetoplastids. Despite compounds being identified which show significant inhibition of the parasite enzymes, this activity does not translate well into cellular and animal models of disease. Understanding to which enzymes antifolates bind under physiol. conditions and how this corresponds to the phenotypic response could provide insight on how to target the folate pathway in these organisms. To facilitate this, the authors have adopted a chem. proteomics approach to study binding of compounds to enzymes of folate metabolism Clin. and literature antifolate compounds were immobilized onto resins to allow for “”pull-down”” of the proteins in the “”folateome””. Using competition studies, proteins which bind the beads specifically and nonspecifically were identified in parasite lysate (Trypanosoma brucei and Leishmania major) for each antifolate compound followed by tryptic digest, Tandem Mass Tag (TMT) labeling of peptides and LC-MS/MS. This method was further exploited by creating a combined folate resin (Folate beads). The resin could pull down upto 9 proteins from the “”folateome””. This information could be exploited to better understand folate metabolism in kinetoplastids and other organisms. In the part of experimental materials, we found many familiar compounds, such as tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6COA of Formula: C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers. Ethers lack the hydroxyl groups of alcohols. Without the strongly polarized O―H bond, ether molecules cannot engage in hydrogen bonding with each other. COA of Formula: C9H19NO4
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem