Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamateIn 2011 ,《Small-molecule hydrophobic tagging-induced degradation of HaloTag fusion proteins》 was published in Nature Chemical Biology. The article was written by Neklesa, Taavi K.; Tae, Hyun Seop; Schneekloth, Ashley R.; Stulberg, Michael J.; Corson, Timothy W.; Sundberg, Thomas B.; Raina, Kanak; Holley, Scott A.; Crews, Craig M.. The article contains the following contents:
The ability to regulate any protein of interest in living systems with small mols. remains a challenge. The authors hypothesized that appending a hydrophobic moiety to the surface of a protein would mimic the partially denatured state of the protein, thus engaging the cellular quality control machinery to induce its proteasomal degradation The authors designed and synthesized bifunctional small mols. to bind a bacterial dehalogenase (the HaloTag protein) and present a hydrophobic group on its surface. Hydrophobic tagging of the HaloTag protein with an adamantyl moiety induced the degradation of cytosolic, isoprenylated and transmembrane HaloTag fusion proteins in cell culture. The authors demonstrated the in vivo utility of hydrophobic tagging by degrading proteins expressed in zebrafish embryos and by inhibiting Hras1G12V-driven tumor progression in mice. Therefore, hydrophobic tagging of HaloTag fusion proteins affords small-mol. control over any protein of interest, making it an ideal system for validating potential drug targets in disease models. The experimental process involved the reaction of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.The C-O bonds that comprise simple ethers are strong. Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate They are unreactive toward all but the strongest bases. Although generally of low chemical reactivity, they are more reactive than alkanes.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem