《A near-infrared excitation/emission fluorescent probe for imaging of endogenous cysteine in living cells and zebrafish》 was written by Xie, Ruihua; Li, Yaqian; Zhou, Zile; Pang, Xiao; Wu, Cuiyan; Yin, Peng; Li, Haitao. Quality Control of 2-Hydroxy-4-methoxybenzaldehyde And the article was included in Analytical and Bioanalytical Chemistry in 2020. The article conveys some information:
The fluorescence imaging technique provides an essential tool for studying biol. systems. However, due to the interference of autofluorescence of biol. tissues, the application of short-wavelength fluorescent probes in biol. imaging was limited. The near-IR (NIR) excitation/emission fluorescent probe possesses unique advantages in optical imaging in vivo, including less light scattering, minimal photo-damage to biol. samples, deep tissue penetration, and weak autofluorescence interference from complicated biol. systems. A convenient fluorophore (E)-2-[2-(6-hydroxy-2,3-dihydro-1H-xanthen-4-yl)vinyl]-3- methylbenzo[d]thiazol-3-ium iodide (DXM-OH) with NIR excitation and emission was rationally designed and developed. What′s more, DXM-OH was applied to construct an “”OFF-ON”” fluorescent probe (E)-2-{2-[6-(acryloyloxy)-2,3-dihydro-1H- xanthen-4-yl]vinyl}-3-methylbenzo[d]thiazol-3-ium iodide (DXM) for sensitive and selective detection of cysteine (Cys). DXM had the advantages of good cell permeability, low toxicity, and excellent optical properties (NIR excitation/emission) and it was successfully applied to image Cys of living cells and zebrafish. In the experiment, the researchers used many compounds, for example, 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Quality Control of 2-Hydroxy-4-methoxybenzaldehyde)
2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Quality Control of 2-Hydroxy-4-methoxybenzaldehyde
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem