Reference of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamateIn 2022 ,《Detecting the insoluble protein aggregates in live cells using an AIE derivative of fluorescent protein chromophore》 appeared in Sensors and Actuators, B: Chemical. The author of the article were Liu, Lihua; Jin, Wenhan; Huang, Yanan; Dai, Jianan; Zheng, Xuwei; Liu, Yu; Ju, Minzi; Shen, Baoxing. The article conveys some information:
Many incurable or unmanageable human protein conformational diseases are associated with the misfolding or aggregation of the aberrantly processed or mutant proteins. In this work, we report an aggregation-induced emission (AIE) derivative of fluorescent protein chromophore used to detect the insoluble protein aggregates in live cells. Based on the 4-hydroxybenzylidene-imidazolinone (HBI), we designed and synthesized a series of AIEgens that span a wide range of viscosity coefficients (χ), thus mimicking the viscous microenvironment of a wide variety of amorphous protein aggregates. The mechanism of these AIEgens were systematically investigated using a combination of photophys. studies, computational analyses and structure-function studies. With the aid of the AggTag method, the optimized probe was used to realize the imaging of the aggregated proteome under the control of the proteostasis network. Besides, we also described the formation and decomposition of protein aggregates under the control of small mol. proteostasis regulators. Briefly, we developed a series of AIEgens that explore varying viscosity sensitivities to visualize protein aggregation in live cells as well as study other biol. processes that associated with local viscosity changes. The results came from multiple reactions, including the reaction of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Reference of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Oxygen is more electronegative than carbon, thus the alpha hydrogens of ethers are more acidic than those of simple hydrocarbons. Reference of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate They are far less acidic than alpha hydrogens of carbonyl groups (such as in ketones or aldehydes), however.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem