《Subtype-Selective Fluorescent Ligands as Pharmacological Research Tools for the Human Adenosine A2A Receptor》 was written by Comeo, Eleonora; Kindon, Nicholas D.; Soave, Mark; Stoddart, Leigh A.; Kilpatrick, Laura E.; Scammells, Peter J.; Hill, Stephen J.; Kellam, Barrie. Related Products of 139115-91-6 And the article was included in Journal of Medicinal Chemistry in 2020. The article conveys some information:
Among class A G protein-coupled receptors (GPCR), the human adenosine A2A receptor (hA2AAR) remains an attractive drug target. However, translation of A2AAR ligands into the clinic has proved challenging and an improved understanding of A2AAR pharmacol. could promote development of more efficacious therapies. Subtype-selective fluorescent probes would allow detailed real-time pharmacol. investigations both in vitro and in vivo. In the present study, two families of fluorescent probes were designed around the known hA2AAR selective antagonist preladenant (SCH 420814). Both families of fluorescent antagonists retained affinity at the hA2AAR, selectivity over all other adenosine receptor subtypes and allowed clear visualization of specific receptor localization through confocal imaging. Furthermore, the Alexa Fluor 647-labeled conjugate allowed measurement of ligand binding affinities of unlabeled hA2AAR antagonists using a bioluminescence resonance energy transfer (NanoBRET) assay. The fluorescent ligands developed here can therefore be applied to a range of fluorescence-based techniques to further interrogate hA2AAR pharmacol. and signaling.tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Related Products of 139115-91-6) was used in this study.
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.Related Products of 139115-91-6Although ethers resist hydrolysis, they are cleaved by hydrobromic acid and hydroiodic acid. Hydrogen chloride cleaves ethers only slowly.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem