In 2012,Tamura, Tomonori; Tsukiji, Shinya; Hamachi, Itaru published 《Native FKBP12 Engineering by Ligand-Directed Tosyl Chemistry: Labeling Properties and Application to Photo-Cross-Linking of Protein Complexes in Vitro and in Living Cells》.Journal of the American Chemical Society published the findings.Application of 139115-91-6 The information in the text is summarized as follows:
The ability to modify target native (endogenous) proteins selectively in living cells with synthetic mols. should provide powerful tools for chem. biol. To this end, the authors recently developed a novel protein labeling technique termed ligand-directed tosyl (LDT) chem. This method uses labeling reagents in which a protein ligand and a synthetic probe are connected by a tosylate ester group. The authors previously demonstrated its applicability to the selective chem. labeling of several native proteins in living cells and mice. However, many fundamental features of this chem. remain to be studied. In this work, the authors investigated the relation between the LDT reagent structure and labeling properties by using native FK506-binding protein 12 (FKBP12) as a target protein. In vitro experiments revealed that the length and rigidity of the spacer structure linking the protein ligand and the tosylate group have significant effects on the overall labeling yield and labeling site. In addition to histidine, which the authors reported previously, tyrosine and glutamate residues were identified as amino acids that are modified by LDT-mediated labeling. Through the screening of various spacer structures, piperazine was optimal for FKBP12 labeling in terms of labeling efficiency and site specificity. Using a piperazine-based LDT reagent containing a photoreactive probe, the authors successfully demonstrated the labeling and UV-induced covalent crosslinking of FKBP12 and its interacting proteins in vitro and in living cells. This study not only furthers the understanding of the basic reaction properties of LDT chem. but also extends the applicability of this method to the investigation of biol. processes in mammalian cells. The results came from multiple reactions, including the reaction of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Application of 139115-91-6)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers. Ethers lack the hydroxyl groups of alcohols. Application of 139115-91-6 Without the strongly polarized O―H bond, ether molecules cannot engage in hydrogen bonding with each other.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem