In 2012,Richards, Sarah-Jane; Jones, Mathew W.; Hunaban, Mark; Haddleton, David M.; Gibson, Matthew I. published 《Probing Bacterial-Toxin Inhibition with Synthetic Glycopolymers Prepared by Tandem Post-Polymerization Modification: Role of Linker Length and Carbohydrate Density》.Angewandte Chemie, International Edition published the findings.Formula: C9H19NO4 The information in the text is summarized as follows:
The authors use tandem post-polymerization modification to obtain glycopolymers with precisely controlled chain length, carbohydrate d., and crucially, defined back-bone-carbohydrate linker lengths. This series of polymers was used to study the multivalent interactions between cholera toxin and peanut agglutinin, to probe the impact of modulating the binding site complementarity on the inhibitory activity of the glycopolymers. This unique combination of structural biol. with materials science gives insights into the cluster glycoside effect and will allow design of active inhibitors. The aim of this investigation was to probe the effect of carbohydrate-binding site accessibility on the measured affinity between multivalent glycopolymers and their target lectins. The B subunit domain of cholera toxin was chosen because it is nontoxic,and a report by Polizzotti and Kiick showed that longer linkers resulted in increased inhibitory activity of cholera toxin. A series of glycopolymers with varying saccharide d., linker length, and chain length were synthesized by tandem post-polymerization modification. Longer linkers were shown to result in increased inhibition of the B subunit of cholera toxin, which is attributed to the depth of the binding pocket. Comparison with peanut agglutinin, which has a shallower binding pocket, revealed no difference in inhibitory activity as a function of linker length. The tandem post-polymerization modification strategy also allowed the effect of carbohydrate d. to be studied. The experimental part of the paper was very detailed, including the reaction process of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Formula: C9H19NO4)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.The C-O bonds that comprise simple ethers are strong. Formula: C9H19NO4 They are unreactive toward all but the strongest bases. Although generally of low chemical reactivity, they are more reactive than alkanes.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem