The author of 《Structural analysis and biological functionalities of iron(III)- and manganese(III)-thiosemicarbazone complexes: in vitro anti-proliferative activity on human cancer cells, DNA binding and cleavage studies》 were Kaya, Busra; Yilmaz, Zehra Kubra; Sahin, Onur; Aslim, Belma; Tukenmez, Ummugulsum; Ulkuseven, Bahri. And the article was published in JBIC, Journal of Biological Inorganic Chemistry in 2019. Formula: C8H8O3 The author mentioned the following in the article:
One iron(III) and two manganese(III) complexes based on thiosemicarbazone were synthesized and characterized using anal. and spectroscopic data. The crystallog. anal. showed the square pyramid structures of the complexes. Electronic spectra anal. was performed to determine the nature of the interaction between the complexes and calf thymus DNA (CT-DNA). DNA cleavage activities of the complexes were examined by gel electrophoresis (pBR322 DNA). The cytotoxicity of the complexes was determined against human cervical carcinoma (HeLa) and human colorectal adenocarcinoma (HT-29) cell lines by MTT assay. The results indicated that complex Fe1(I) is bound to CT-DNA via the intercalation mode, while complexes Mn1(II) and Mn2(III) are bound to CT-DNA via groove binding and/or electrostatic interactions rather than the intercalation mode. In addition, they showed good binding activity, which followed the order of I > III > II. Complexes were found to promote the cleavage of DNA from supercoiled form (SC, Form I) to nicked circular form (NC, Form II) without concurrent formation of Form III, revealing the single-strand DNA cleavage. No significant cleavage was found in the presence of II and III; however, it was observed at 2000 and 3000μM concentrations of I. The ability of I to cleave DNA was greater than that of other complexes and these results are in conformity with their DNA-binding affinities. Cytotoxicity determination tests revealed that the complex I on HeLa and HT-29 cells exhibited a higher anti-proliferative effect than II and III (I > III > II). These studies suggested that the complex I could be a good candidate as a chemotherapeutic drug targeting DNA. In the experiment, the researchers used many compounds, for example, 2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3Formula: C8H8O3)
2-Hydroxy-4-methoxybenzaldehyde(cas: 673-22-3) is the main component of root bark essential oil of Periploca sepium Bunge. It is a potential tyrosinase inhibitor present in African medicinal plants. 2-Hydroxy-4-methoxybenzaldehyde was used in the synthesis of Schiff base ligand.Formula: C8H8O3
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem