Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamateIn 2015 ,《Covalent Protein Labeling by Enzymatic Phosphocholination》 appeared in Angewandte Chemie, International Edition. The author of the article were Heller, Katharina; Ochtrop, Philipp; Albers, Michael F.; Zauner, Florian B.; Itzen, Aymelt; Hedberg, Christian. The article conveys some information:
We present a new protein labeling method based on the covalent enzymic phosphocholination of a specific octapeptide amino acid sequence in intact proteins. The bacterial enzyme AnkX from Legionella pneumophila has been established to transfer functional phosphocholine moieties from synthetically produced CDP-choline derivatives to N-termini, C-termini, and internal loop regions in proteins of interest. Furthermore, the covalent modification can be hydrolytically removed by the action of the Legionella enzyme Lem3. Only a short peptide sequence (eight amino acids) is required for efficient protein labeling and a small linker group (PEG-phosphocholine) is introduced to attach the conjugated cargo. In addition to this study using tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate, there are many other studies that have used tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate) was used in this study.
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers. Ethers lack the hydroxyl groups of alcohols. Safety of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate Without the strongly polarized O―H bond, ether molecules cannot engage in hydrogen bonding with each other.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem