In 2012,Fujishima, Sho-hei; Yasui, Ryosuke; Miki, Takayuki; Ojida, Akio; Hamachi, Itaru published 《Ligand-Directed Acyl Imidazole Chemistry for Labeling of Membrane-Bound Proteins on Live Cells》.Journal of the American Chemical Society published the findings.SDS of cas: 139115-91-6 The information in the text is summarized as follows:
Chem.-based protein labeling in living cells is undoubtedly useful for understanding natural protein functions and for biol./pharmaceutical applications. Here, the authors report a novel approach for endogenous membrane-bound protein labeling for both in vitro and live cell conditions. A moderately reactive alkyloxyacyl imidazole (AI) assisted by ligand-binding affinity (ligand-directed AI (LDAI)) chem. allowed the authors to selectively modify natural proteins, such as dihydrofolate reductase (DHFR) and folate receptor (FR), neither of which could be efficiently labeled using the recently developed ligand-directed tosylate approach. It was clear that LDAI selectively labeled a single Lys(K32) in DHFR, proximal to the ligand-binding pocket. The authors also demonstrate that the fluorescein-labeled (endogenous, by LDAI) FR works as a fluorescent biosensor on the live KB cell surface, which allowed the authors to carry out unprecedented in situ kinetic anal. of ligand binding to FR. The experimental part of the paper was very detailed, including the reaction process of tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6SDS of cas: 139115-91-6)
tert-Butyl (2-(2-hydroxyethoxy)ethyl)carbamate(cas: 139115-91-6) belongs to ethers.The C-O bonds that comprise simple ethers are strong. SDS of cas: 139115-91-6 They are unreactive toward all but the strongest bases. Although generally of low chemical reactivity, they are more reactive than alkanes.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem