Spadaro, Angelo; Rao, Marco; Lorenti, Miriam; Romano, Mario Rosario; Augello, Antonio; Eandi, Chiara Maria; Platania, Chiara Bianca Maria; Drago, Filippo; Bucolo, Claudio published the artcile< New brilliant blue G derivative as pharmacological tool in retinal surgery>, Formula: C3H7BrO, the main research area is alkyl brilliant blue inner limiting membrane retina vitreoretinal surgery; Brilliant Blue G; drug design; inner limiting membrane; pucker; retina; vitreoretinal surgery.
Our study was aimed at assessing the retinal binding of a new synthetic Brilliant Blue G (BBG) derivative (pure benzyl-Brilliant Blue G; PBB) ophthalmic formulation, to improve vitreoretinal surgery procedure. Protein affinity of the new mol. was evaluated in vitro (cell-free assay) and in silico. Furthermore, an ex vivo model of vitreoretinal surgery was developed by using porcine eyes to assess the pharmacol. profile of PBB, compared to com. formulations based on BBG and methyl-BBG (Me-BBG). PBB showed a higher affinity for proteins (p < 0.05), compared to BBG and Me-BBG. In vitro and in silico studies demonstrated that the high selectivity of PBB could be related to high lipophilicity and binding affinity to fibronectin, the main component of the retinal internal limiting membrane (ILM). The PBB staining capabilities were evaluated in porcine eyes in comparison with BBG and Me-BBG. Forty microliters of each formulation were slowly placed over the retinal surface and removed after 30 s. After that, ILM peeling was carried out, and the retina collected. BBG, Me-BBG, and PBB quantification in ILM and retina tissues was carried out by HPLC anal. PBB levels in the ILM were significantly (p < 0.05) higher compared to BBG and Me-BBG formulations. On the contrary, PBB showed a much lower (p < 0.05) distribution in retina (52 ng/mg tissue) compared to BBG and Me-BBG, in particular PBB levels were significantly (p < 0.05) lower. Therefore, the new synthetic Brilliant Blue derivative (PBB) showed a great ILM selectivity in comparison to underneath retinal layers. In conclusion, these findings had high translational impact with a tangible improving in ex vivo model of retinal surgery, suggesting a future use during surgical practice. Frontiers in Pharmacology published new progress about Bovine serum albumin Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6482-24-2 belongs to class ethers-buliding-blocks, and the molecular formula is C3H7BrO, Formula: C3H7BrO.
Referemce:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem