Iacazio, Gilles;Perissol, Claude;Faure, Bruno published 《A new tannase substrate for spectrophotometric assay》. The research results were published in《Journal of Microbiological Methods》 in 2000.Product Details of 2235-01-0 The article conveys some information:
A new tannase substrate, protocatechuic acid p-nitrophenyl ester, I, was synthesized using modern synthetic methods. The synthesis was designed to be performed by non-specialized chemists. It only involves four steps, three of which are protection-deprotection, and uses standard methods of separation and purification, such as recrystallization and column chromatog. over silica. Under tannase action, protocatechuic acid p-nitrophenyl ester, I, releases p-nitrophenol, which is easily measured spectrophotometrically either at 350 nm for pH values < 6 or at 400 nm for pH values of 6-7 (yellow). The pH-response and the catalytic parameters of a crude Penicillium sp. tannase preparation were determined using I as substrate, thus showing the usefulness of this substrate in determining tannase activity. To complete the study, the researchers used Dimethoxydiphenylmethane (cas: 2235-01-0) .
The unique properties of ethers (i.e., that they are strongly polar, with nonbonding electron pairs but no hydroxyl group) enhance the formation and use of many reagents. For example, Grignard reagents cannot form unless an ether is present to share its lone pair of electrons with the magnesium atom.Product Details of 2235-01-0
Reference:
Ether – Wikipedia,
Ether | (C2H5)2O – PubChem