Let¡¯s face it, organic chemistry can seem difficult to learn, Product Details of 645-36-3, Especially from a beginner¡¯s point of view. Like 645-36-3, Name is 2,2-Diethoxyethanamine, molecular formula is C14H8N2S4, belongs to thiazoles compound. In a document, author is Zheng, Dan, introducing its new discovery.
NLRP3 inflammasome-mediated endothelial cells pyroptosis is involved in decabromodiphenyl ethane-induced vascular endothelial injury
Decabromodiphenyl ethane (DBDPE) is a novel environmental pollutant that has attracted growing attention. Previous studies have indicated that DBDPE could induce vascular endothelial injury and cardiovascular damage, but the underlying mechanisms are not well understood. This study was designed to examine the mechanisms of DBDPE induces vascular endothelial injury. In vivo, Sprague-Dawley rats were administered with 0, 5, 50, 500 mg/kg bw/day of DBDPE via gavage for 28 days. Results showed that DBDPE could damage abdominal aortas morphological and ultrastructural structure and increase the protein levels of interleukin 1 beta (IL-1 beta) and interleukin 18 (IL-18) of the abdominal aortas. Moreover, DBDPE induced NLRP3 inflammasome activation and activated caspase-1 in abdominal aorta endothelium of rats. In vitro, human vascular endothelial cells (HAECs) were treated with different concentrations of DBDPE (0, 6.25, 12.5, 25, 50, and 100 mu M). DBDPE not only induced cytotoxicity and reactive oxygen species (ROS) generation in HAECs but also caused HAECs pyroptosis, which was evidenced by the elevated expression of Nod-like receptor protein -3 (NLRP3), ASC, and caspase-1 in DBDPE-treated group. To further elucidate the effects of NLRP3 inflammasome on DBDPE-induced HAECs pyroptosis, we constructed NLRP3 knockdown HAECs by lentivirus-mediated short hairpin RNA (shRNA). And the results showed that NLRP3 knockdown downregulated DBDPE-induced increases of caspase-1 activity and caspase-1, ASC and NLRP3 mRNA and protein expression levels. Accordingly, our data suggested that DBDPE may damage vascular endothelium by NLRP3 inflammasome-mediated endothelial cells pyroptosis. (C) 2020 Elsevier Ltd. All rights reserved.
If you are hungry for even more, make sure to check my other article about 645-36-3, Product Details of 645-36-3.